Full-Service Sanger Sequencing
The CCG staff will perform the sequencing:
- Submit templates Monday and Wednesday afternoons by 4:30 pm.
- Data will be available for download the following afternoon.
- Bring templates to 232 BB (-20 freezer) in a 0.5 ml tube.
NGS Library Preparation for Illumina® HiSeq 4000
- Illumina® TruSeq Stranded mRNA
- Illumina® TruSeq Stranded Total RNA
- Kapa Hyper Prep
- RNA QC
Integrity of RNA by Bio-Rad Experion Electrophoresis System
Quantification by Fluorometer/RiboGreen Assay
Purity of RNA by NanoDrop One
- RNA Extraction from tissues and cells
- RNA Cleanup and Concentration
- RNA Amplification
- cDNA Normalization
- DNA QC
Quantification by Fluorometer/PicoGreen Assay
Purity of DNA by NanoDrop One
- gDNA Isolation from tissues and cells
- gDNA Cleanup and Concentration
- Custom PCR Projects