CCG Services

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CCG Services

Sanger Sequencing

Full-Service Sanger Sequencing
The CCG staff is currently performing full-service Sanger sequencing on Tuesdays and Thursdays:

  • Submit templates by 6am on Tuesdays and Thursdays for runs that day. Data will be available for download either later that day or the following morning.
  • The cost of each sample will be $6.75 effective March 1, 2021.
  • Bring templates to 232 BB (-20 freezer) in a 0.5 ml tube.

Instrument Scheduling and Sequencing Results Login
Sequence Submission Form
Template and Primer Requirements

NGS Library Preparation for Illumina® NovaSeq or Illumina® iSeq

  • Contact Cindy Toll (cindy-toll@uiowa.edu) to discuss library preparation options and to get a customized quote for your project.

RNA Services

  • RNA QC
              Integrity of RNA using capillary electrophoresis (Agilent BioAnalyzer or Bio-Rad Experion)
              Quantification by Fluorometer
              Purity of RNA using NanoDrop One
  • RNA Extraction from tissues and cells
  • RNA Cleanup and Concentration

DNA Services

  • DNA QC
              Integrity of DNA using capillary electrophoresis (Agilent BioAnalyzer or Bio-Rad Experion)
              Quantification by Fluorometer
              Purity of DNA using NanoDrop One
  • gDNA Isolation from tissues and cells
  • gDNA Cleanup and Concentration
  • Custom Projects
              PCR
              Primer Design
              Cloning
              Sanger Sequencing