College of Liberal Arts & Sciences
CCG Services
CCG Services
Sanger Sequencing
Full-Service Sanger Sequencing
The CCG staff is currently performing full-service Sanger sequencing on Tuesdays and Thursdays:
- Submit templates by 8am on Tuesdays and Thursdays for runs that day. Data will be available for download either later that day or the following morning.
- The cost of each sample will be $6.75 effective March 1, 2021.
- Bring templates to 232 BB (-20 freezer) in a 0.5 ml tube.
Instrument Scheduling and Sequencing Results Login
Sequence Submission Form
Template and Primer Requirements
NGS Library Preparation for Illumina® NovaSeq or Illumina® iSeq
- Contact Christine Kondratick (christine-kondratick@uiowa.edu) to discuss library preparation options and to get a customized quote for your project.
RNA Services
- RNA QC
Integrity of RNA using capillary electrophoresis (Agilent BioAnalyzer or Bio-Rad Experion)
Quantification by Fluorometer
Purity of RNA using NanoDrop One - RNA Extraction from tissues and cells
- RNA Cleanup and Concentration
DNA Services
- DNA QC
Integrity of DNA using capillary electrophoresis (Agilent BioAnalyzer or Bio-Rad Experion)
Quantification by Fluorometer
Purity of DNA using NanoDrop One - gDNA Isolation from tissues and cells
- gDNA Cleanup and Concentration
- Custom Projects
PCR
Primer Design
Cloning
Sanger Sequencing