CCG Services

Sanger Sequencing

Full-Service Sanger Sequencing
The CCG staff is currently performing full-service Sanger sequencing on Tuesdays and Thursdays:

• Submit templates by 6am on Tuesdays and Thursdays for runs that day. Data will be available for download either later that day or the following morning.
• The cost of each sample will be $6.75 effective March 1, 2021.
• Bring templates to 232 BB (-20 freezer) in a 0.5 ml tube.

Instrument Scheduling and Sequencing Results Login
Sequence Submission Form
Template and Primer Requirements

NGS Library Preparation for Illumina® NovaSeq or Illumina® iSeq

• Contact Christine Kondratick (christine-kondratick@uiowa.edu) to discuss library preparation options and to get a customized quote for your project.

RNA Services

• RNA QC
            Integrity of RNA using capillary electrophoresis (Agilent BioAnalyzer or Bio-Rad Experion)
            Quantification by Fluorometer
            Purity of RNA using NanoDrop One
• RNA Extraction from tissues and cells
• RNA Cleanup and Concentration

DNA Services

• DNA QC
            Integrity of DNA using capillary electrophoresis (Agilent BioAnalyzer or Bio-Rad Experion)
            Quantification by Fluorometer
            Purity of DNA using NanoDrop One
• gDNA Isolation from tissues and cells
• gDNA Cleanup and Concentration
• Custom Projects
            PCR
            Primer Design
            Cloning
            Sanger Sequencing