College of Liberal Arts & Sciences
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Cells from the non-cancerous breast epithelial cell line MCF-10A grown in 3D Matrigel divide to form islands of cells (primary aggregates) that do not undergo coalescence. This movie is a series of DIC images from a single plane acquired at 30 minute intervals over a period of 70 hours.
Cells from the tumorigenic cell line MoVi-10´ undergo rapid coalescence when embedded in 3D Matrigel matrix. This movie is a time series of DIC images at a single plane, acquired at 30 minute intervals over a period of 70 hours.
J3D-DIAS-4.2 generated 4D reconstruction of the non-cancerous breast epithelial cell line MCF-10A cultured in 3D Matrigel demonstrates that the cell islands do not coalesce.
J3D-DIAS4.2 generated 4D reconstruction of the tumorigenic cell line MoVi-10´ cultured in 3D Matrigel demonstrates that, in contrast to normal cells, aggressively tumorigenic cancer cells rapidly coalesce.