Cells from the non-cancerous breast epithelial cell line MCF-10A grown in 3D Matrigel divide to form islands of cells (primary aggregates) that do not undergo coalescence.  This movie is a series of DIC images from a single plane acquired at 30 minute intervals over a period of 70 hours.

Cells from the tumorigenic cell line MoVi-10´ undergo rapid coalescence when embedded in 3D Matrigel matrix. This movie is a time series of DIC images at a single plane, acquired at 30 minute intervals over a period of 70 hours.

J3D-DIAS-4.2 generated 4D reconstruction of the non-cancerous breast epithelial cell line MCF-10A cultured in 3D Matrigel demonstrates that the cell islands do not coalesce.

J3D-DIAS4.2 generated 4D reconstruction of the tumorigenic cell line MoVi-10´  cultured in 3D Matrigel demonstrates that, in contrast to normal cells, aggressively tumorigenic cancer cells rapidly coalesce.